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. 2013:2013:294789.
doi: 10.1155/2013/294789. Epub 2013 Sep 9.

Electroacupuncture Stimulation at CV12 Inhibits Gastric Motility via TRPV1 Receptor

Zhi Yu  1 , Xin Cao, Youbing Xia, Binbin Ren, Hong Feng, Yali Wang, Jingfeng Jiang, Bin Xu
Affiliations

Electroacupuncture Stimulation at CV12 Inhibits Gastric Motility via TRPV1 Receptor

Zhi Yu et al. Evid Based Complement Alternat Med. 2013.

Abstract

Gastric dysmotility is one of the major pathophysiological factors in functional gastrointestinal disorders. Acupuncture, as one of the alternative approaches, is efficacious in the treatment of gastrointestinal motility disorders; however, the mechanism underlying its action is unclear. In the present study, we used both capsazepine, a TRPV1 antagonist, and TRPV1 knockout mice. Animals were divided into wild-type group (WT), capsazepine injection group (CZP, 0.5 mg/kg, i.p.), and TRPV1 knockout mice group (TRPV1(-/-)). Each of these three groups was divided into three subgroups, which were subjected to EA stimulation at acupoint Zhongwan (CV12) at a different intensity (1, 2, or 4 mA). We demonstrated that electroacupuncture at Zhongwan (CV12) markedly inhibited gastric motility at 2 and 4 mA in an intensity-dependent manner in wild-type mice. The inhibitory effect was also observed in capsazepine-injected and TRPV1(-/-) mice but was no longer intensity dependent, indicating that TRPV1 is partially involved in the electroacupuncture-mediated modulation of gastric motility.

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Figures

Figure 1
Figure 1
Experimental procedure. (a) Time scale of stimulation in the wild-type and TRPV1−/− groups. (b) Time scale of stimulations in the capsazepine (CZP) injection group.
Figure 2
Figure 2
Gastric response to electroacupuncture (EA) stimulation at CV12 in wild-type mice. (a) Significant inhibition of gastric motility was induced by EA stimulation at intensities 2 mA (n = 10) and 4 mA (n = 10). ∗∗: Versus baseline, P < 0.001. (b)–(d) Representative examples of EA stimulation at CV12 with different intensities.
Figure 3
Figure 3
Gastric responses to electroacupuncture (EA) stimulation at CV12 after capsazepine (CZP) injection. (a) Significant inhibition of gastric motility was induced by EA at intensities 2 mA (n = 10) and 4 mA (n = 10). ∗∗: Versus CZP, P < 0.001. (b)–(d) Representative examples of EA stimulation at CV12 with different intensities.
Figure 4
Figure 4
Gastric responses to electroacupuncture (EA) stimulation at CV12 in TRPV1−/− mice. (a) Significant inhibition of gastric motility was induced by EA at intensities 2 mA (n = 10) and 4 mA (n = 10). ∗∗: Versus baseline, P < 0.001. (b)–(d) Representative examples of EA stimulation at CV12 with different intensities. (e) Percentage inhibition of gastric motility after EA stimulation at CV12 with different intensities in all three experimental groups. #: Versus WT, P < 0.05; ##: Versus WT, P < 0.01; ΔΔ: Versus 1 mA, P < 0.001; ★: Versus 2 mA, P < 0.05.
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