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. 2013 Oct 10:3:2847.
doi: 10.1038/srep02847.

Live pigs produced from genome edited zygotes

Simon G Lillico  1 , Chris Proudfoot, Daniel F Carlson, Dana Stverakova, Claire Neil, Carol Blain, Tim J King, William A Ritchie, Wenfang Tan, Alan J Mileham, David G McLaren, Scott C Fahrenkrug, C Bruce A Whitelaw
Affiliations

Live pigs produced from genome edited zygotes

Simon G Lillico et al. Sci Rep. .

Abstract

Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any chosen mating.

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Conflict of interest statement

The authors declare no competing financial interests.

Figures

Figure 1
Figure 1
A. Location of TALEN, ZFN and PCR primers for sequencing amplicon. Porcine RELA sequence 769417-769110 accession number NW_003609513.1. B. Example Cel1 Assay. Lanes A2, A12, B6, B14 and B20 were identified as having cleavage products consistent with editing events at the target site. Direct sequencing of the same PCR products confirmed this interpretation and identified B12, B16, B17 and B18 as having only wild-type sequence.
See this image and copyright information in PMC

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