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. 2014 Aug;102(8):2704-12.
doi: 10.1002/jbm.a.34942. Epub 2013 Sep 24.

Mechanical and biological properties of chitosan/carbon nanotube nanocomposite films

Ashkan Aryaei  1 , Ahalapitiya H Jayatissa, Ambalangodage C Jayasuriya
Affiliations

Mechanical and biological properties of chitosan/carbon nanotube nanocomposite films

Ashkan Aryaei et al. J Biomed Mater Res A. 2014 Aug.

Abstract

In this article, different concentrations of multiwalled carbon nanotube (MWCNT) were homogeneously dispersed throughout the chitosan (CS) matrix. A simple solvent-cast method was used to fabricate chitosan films with 0.1, 0.5, and 1% of MWCNT with the average diameter around 30 nm. The CS/MWCNT films were characterized for structural, viscous and mechanical properties with optical microscopy, wide-angle X-ray diffraction, Raman spectroscopy, tensile test machine, and microindentation testing machine. Murine osteoblasts were used to examine the cell viability and attachment of the nanocomposite films at two time points. In comparison to the pure chitosan film, the mechanical properties, including the tensile modulus and strength of the films, were greatly improved by increasing the percentage of MWCNT. Furthermore, adding MWCNT up to 1% increased the viscosity of the chitosan solution by 15%. However, adding MWCNT decreased the samples ductility and transparency. In biological point of view, no toxic effect on osteoblasts was observed in the presence of different percentages of MWCNT at day 3 and day 7. This investigation suggested MWCNT could be a promising candidate for improving chitosan mechanical properties without inducing remarkable cytotoxicity on bone cells.

Keywords: chitosan; cytotoxicity; mechanical properties; multiwalled carbon nanotube; nanocomposite.

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Conflict of interest statement

Conflict of interest statement

We declare that none of authors involved in this research work have conflict of interest.

Figures

Figure 1
Figure 1
(A) CS/MWCNT nanocomposite films with different percentages of MWCNT after 3 days in a stationary position. (B) Optical microscopy images and (C) normal camera images of CS/MWCNT films with different concentrations of MWCNT.
Figure 2
Figure 2
XRD patterns of CS/MWCNT films with different concentrations of MWCNT.
Figure 3
Figure 3
(A) Transmittance curves for CS films with different concentrations of MWCNT. (B) Absorbance of CS/MWCNT films as a function of elative concentration of MWCNTs.
Figure 4
Figure 4
Raman spectra of CS films with different MWCNT concentrations and MWCNT powder.
Figure 5
Figure 5
pH value changing of CS/MWCNT films with (A) initial pH value of 7.4, (B) initial pH value of 5.
Figure 6
Figure 6
(A) Typical injection curves obtained for CS/MWCNT pastes with different percentages of MWCNT. (B) Relationship between viscosity and MWCNT concentration.
Figure 7
Figure 7
(A) Tensile test of CS/MWCNT films. (B) The effect of MWCNT concentration on Young’s modulus and ductility of samples.
Figure 8
Figure 8
Microhardness of CS/MWCNT films at 10 gf load as a function of MWCNT concentration.
Figure 9
Figure 9
Fluorescent microscopy images of osteoblasts (A) after 3 days and (B) after 7 days treated with Live/Dead assay.
See this image and copyright information in PMC

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