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. 1975 Jun;147(3):473-7.
doi: 10.1042/bj1470473.

Preparation of polyribosome aminoacyl-transfer ribonucleic acid from the muscle of chick embryos

M Nwagwu

Preparation of polyribosome aminoacyl-transfer ribonucleic acid from the muscle of chick embryos

M Nwagwu. Biochem J. 1975 Jun.

Abstract

A procedure for preparing polyribosome aminoacyl-tRNA free from contamination by supernatant aminoacyl-tRNA and free amino acids is described. Important features of the procedure are the use of acidic buffers to help protect the amino acid-tRNA linkage and the inclusion of sodium dodecyl sulphate, to inhibit ribonuclease activity. The specific radioactivity of polyribosome aminoacyl-tRNA is high within 30s and reaches a maximum in 2 1/2 min, well ahead of polyribosome peptides which, as described by Herrmann et al. (1971), attain maximum specific radioactivity in about 10 min.

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