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. 2014:1072:725-35.
doi: 10.1007/978-1-62703-631-3_50.

Immunoproteomics analysis of food allergens

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Immunoproteomics analysis of food allergens

Rika Nakamura et al. Methods Mol Biol. 2014.

Abstract

Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, because it is a simple and inexpensive tool for rapid identification of several IgE-binding proteins. In this section, we describe our protocols for identification of food allergens using immunoproteomics and discuss a few technical points in detail.

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