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Review
. 2013 Oct 16:4:144.
doi: 10.3389/fendo.2013.00144.

Glutamine synthetase as an astrocytic marker: its cell type and vesicle localization

Enrico Anlauf  1 , Amin Derouiche
Affiliations
Review

Glutamine synthetase as an astrocytic marker: its cell type and vesicle localization

Enrico Anlauf et al. Front Endocrinol (Lausanne). .
No abstract available

Keywords: astrocyte; deconvolution; glutamate metabolism; immunocytochemistry; oligodendrocyte.

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Figures

Figure 1
Figure 1
Organelle-bound localization of GS-immunoreactivity, in primary culture of rat cortical astrocytes using the following anti-GS antibodies: (1) polyclonal made in rabbit (15), or (2) goat (Santa Cruz sc-6640), or (3) mouse monoclonal (Chemicon-Millipore, Billerica, MA, USA; clone GS-6, MAB 302). (A–J) Controls for double-labeling (red and green) with two anti-GS antibodies, red channel always in left, green in right column. (A,B) Control for autofluorescence, no immunoreagents. (C,D) Control for fluorescence red-to-green bleed through: Single staining anti-GS (1) with secondary anti-rabbit antibody (red). (E,F) Control for detection system of green channel: Same as in (C,D), in addition secondary anti-mouse antibody (green). (G,H) Control for fluorescence green-to-red bleed through: Single staining anti-GS (3) with secondary anti-mouse antibody (green). (I,J) control for detection system of red channel: Same as in (G,H), in addition anti-rabbit antibody (red). (K) Double-labeling by antibodies (1) and (2) coincides on the same organelles, even at high magnification [(N), from inset in (K)]. Antibody (1), red channel, labels the complete outline of organelles, whereas antibody (2), green channel only yields pixels within the extent of individual red labeled structures. Optical section 100 μm thick, after deconvolution. Single labeling by antibodies (3) (L) or (2) [(M,O), from inset] yields comparable organelles. Scale 5 μm [in (J), for (A–J)], 3 μm (K,M), 4 μm (L), 0.25 μm (N), 0.5 μm (O).
See this image and copyright information in PMC

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