Molecular characterization of Cryptosporidium spp. from foals in Italy
- PMID: 24138936
- DOI: 10.1016/j.tvjl.2013.09.004
Molecular characterization of Cryptosporidium spp. from foals in Italy
Abstract
Fourteen Cryptosporidium isolates from hospitalized foals were genotyped and subtyped using PCR-RFLP analysis of the 18S rDNA. Cryptosporidium parvum and Cryptosporidium horse genotype were detected in 3 and 11 stool specimens, respectively. Sequences of the gp60 gene of Cryptosporidium horse genotype allowed identification of the subtype family VIaA15G4, defining a novel microsatellite pattern within horse subtype VIa. Cryptosporidium horse genotype has only been described occasionally worldwide and this is the first time it has been identified in foals from Italy.
Keywords: 18S rRNA; Cryptosporidium horse genotype; Cryptosporidium parvum; Equine; gp60.
Copyright © 2013 Elsevier Ltd. All rights reserved.
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