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. 2013 Nov 20;61(46):10960-70.
doi: 10.1021/jf401802n. Epub 2013 Nov 5.

Profiling polyphenols in five Brassica species microgreens by UHPLC-PDA-ESI/HRMS(n.)

Jianghao Sun  1 , Zhenlei Xiao, Long-Ze Lin, Gene E Lester, Qin Wang, James M Harnly, Pei Chen
Affiliations

Profiling polyphenols in five Brassica species microgreens by UHPLC-PDA-ESI/HRMS(n.)

Jianghao Sun et al. J Agric Food Chem. .

Abstract

Brassica vegetables are known to contain relatively high concentrations of bioactive compounds associated with human health. A comprehensive profiling of polyphenols from five Brassica species microgreens was conducted using ultrahigh-performance liquid chromatography photodiode array high-resolution multistage mass spectrometry (UHPLC-PDA-ESI/HRMS(n)). A total of 164 polyphenols including 30 anthocyanins, 105 flavonol glycosides, and 29 hydroxycinnamic acid and hydroxybenzoic acid derivatives were putatively identified.The putative identifications were based on UHPLC-HRMS(n) analysis using retention times, elution orders, UV-vis and high-resolution mass spectra, and an in-house polyphenol database as well as literature comparisons. This study showed that these five Brassica species microgreens could be considered as good sources of food polyphenols.

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Figures

Figure 1
Figure 1
Basic chemical structures identified from five Brassica species microgreens.
Figure 2
Figure 2
The UHPLC chromatogram from five Brassica species microgreens: red cabbage (A), purple kohlrabi (B), red mustard (C) and purple mustard (D) under 520 nm.
Figure 3
Figure 3
The UHPLC chromatogram of five Brassica species microgreens: red cabbage (A), purple kohlrabi (B), red mustard (C), purple mustard (D) and mizuna (E) under 330 nm.
See this image and copyright information in PMC

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