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. 2013 May 16;10(4):179-85.
doi: 10.4314/ajtcam.v10i4.29. eCollection 2013.

Applied orthogonal experiment design for the optimum microwave-assisted extraction conditions of polysaccharides from Rhodiolae Radix

Fenglin Li  1 , Furen Xiao, Jingli Gong, Tian Yu
Affiliations

Applied orthogonal experiment design for the optimum microwave-assisted extraction conditions of polysaccharides from Rhodiolae Radix

Fenglin Li et al. Afr J Tradit Complement Altern Med. .

Abstract

An experiment on polysaccharides from Rhodiolae Radix (PRR) extraction was carried out using microwave-assisted extraction (MAE) method with an objective to establishing the optimum MAE conditions of PRR. Single factor experiments were performed to determine the appropriate range of extraction conditions, and the optimum conditions were obtained using orthogonal experiment design. The results showed that the optimum MAE conditions of PRR were as follows: solid-liquid ratio of 1:45 g/mL, irradiation power of 480 W, and irradiation time of 8 min, while extraction yield of PRR was 3.24 %.

Keywords: Rhodiolae Radix; microwave-assisted extraction; orthogonal experiment design; polysaccharides.

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Figures

Figure 1
Figure 1
Standard curve of glucose
Figure 2
Figure 2
Effect of solid-liquid ratio on extraction yield of polysaccharides from Rhodiolae Radix
Figure 3
Figure 3
Effect of irradiation power on extraction yield of polysaccharides from Rhodiolae Radix
Figure 4
Figure 4
Effect of irradiation power on extraction yield of polysaccharides from Rhodiolae Radix
See this image and copyright information in PMC

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