Acetylcholine responses in snail neurons: increase and decrease in potassium conductance succeeding inward currents

Abstract

In the identified neurons B1 and B3 of the buccal ganglion of Helix pomatia, the initial acetylcholine (ACh) inward current was succeeded by two types of secondary responses. The secondary responses consisted either in an outward current or in a long-lasting inward current or in a combination of both. The secondary outward current was decreased with membrane hyperpolarization, associated with a decrease of membrane resistance and abolished in Ca2+-free Co2+ solution. It is assumed to be a K+ current activated by an influx of Ca2+. The secondary inward current also decreased with membrane hyperpolarization, but was associated with an increase of the membrane resistance and could be mimicked by an injection of Na+ into the cells. It is suggested to be due to a block of K+ channels by intracellular Na+. When the secondary responses appeared combined, the outward current preceded the inward current.