doi: 10.1139/g85-093.
Synchronization of human lymphocyte cultures by fluorodeoxyuridine
- PMID: 2415229
- DOI: 10.1139/g85-093
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Synchronization of human lymphocyte cultures by fluorodeoxyuridine
Can J Genet Cytol.
1985 Oct.
Abstract
A simple technique for synchronization of human lymphocyte cultures with fluorodeoxyuridine (FudR) is presented. The S-phase block induced by the FudR is released by simultaneous exposure to 5-bromodeoxyuridine (BrdU) and Hoechst 33258 or by thymidine and Hoechst 33258. This method provides a high mitotic index with high percentage of prometaphase chromosomes. This simple method is highly advantageous and easy to utilize in clinical cytogenetics.
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