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. 1985 Oct;27(5):622-5.
doi: 10.1139/g85-093.

Synchronization of human lymphocyte cultures by fluorodeoxyuridine

M De Braekeleer, M Keushnig, C C Lin

Synchronization of human lymphocyte cultures by fluorodeoxyuridine

M De Braekeleer et al. Can J Genet Cytol. 1985 Oct.

Abstract

A simple technique for synchronization of human lymphocyte cultures with fluorodeoxyuridine (FudR) is presented. The S-phase block induced by the FudR is released by simultaneous exposure to 5-bromodeoxyuridine (BrdU) and Hoechst 33258 or by thymidine and Hoechst 33258. This method provides a high mitotic index with high percentage of prometaphase chromosomes. This simple method is highly advantageous and easy to utilize in clinical cytogenetics.

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