Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Sep;86(9):1590-3.
doi: 10.1002/jmv.23821. Epub 2013 Oct 24.

Phylogenetic characterization of enterovirus 68 strains in patients with respiratory syndromes in Italy

Antonio Piralla  1 , Alessia Girello, Michela Grignani, Monica Gozalo-Margüello, Antonietta Marchi, Gianluigi Marseglia, Fausto Baldanti
Affiliations

Phylogenetic characterization of enterovirus 68 strains in patients with respiratory syndromes in Italy

Antonio Piralla et al. J Med Virol. 2014 Sep.

Abstract

Enterovirus 68 (EV-D68) was associated with mild to severe respiratory infections. In the last 4 years, circulation of different EV-D68 strains has been documented worldwide. In this study, the phylogenetic characterization of nine EV-D68 strains identified in patients in the 2010-2012 period and 12 additional EV-D68 Italian strains previously identified in 2008 in Italy was described. From January 2010 to December 2012, a total of 889 respiratory specimens from 588 patients stayed or visited at the Fondazione IRCCS Policlinico San Matteo were positive for HRV or HEV. Extracted nucleic acids were amplified by one-step RT-PCR with primer specific for VP1 region of EV-D68 and purified positive PCR products were directly sequenced. Overall, 9/3736 (0.24%) patients were EV-D68 positive. Of these, 7/9 (77.8%) were pediatric and two (22.2%) were adults. Five out of seven (71.4%) pediatric patients had lower respiratory tract infection with oxygen saturation <94%. Four cases were detected from August through October 2010, while five other cases from September through December 2012. The Italian EV-D68 strains in 2008 belonged to clade A (n = 5) and clade C (n = 7). In 2010 all the Italian strains belonged to clade A (n = 4) and in 2012, four Italian strains belonged to clade B and one to clade A. In conclusion, we provide additional evidence supporting a role of EV-D68 in severe respiratory infection in pediatric patients. In addition, all the three EV-D68 clades circulating worldwide were identified in Italy in a 5-year period of time.

Keywords: Italian strains; VP1 sequences; enterovirus 68; respiratory infection.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Phylogenetic tree based on the alignment of complete VP1 sequences of EV‐D68. The aLRT values >0.70 are indicated at nodes. Italian strains are reported in red. The EV‐D68 prototype strain Fermon is reported in italics. The reference strains are reported with accession number, country of origin, and year of collection.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O. 2010. New algorithms and methods to estimate maximum‐likelihood phylogenies: Assessing the performance of PhyML 3.0. Syst Biol 59:307–321. - PubMed
    1. Ikeda T, Mizuta K, Abiko C, Aoki Y, Itagaki T, Katsushima F, Katsushima Y, Matsuzaki Y, Fuji N, Imamura T, Oshitani H, Noda M, Kimura H, Ahiko T. 2012. Acute respiratory infections due to enterovirus 68 in Yamagata, Japan between 2005 and 2010. Microbiol Immunol 56:139–143. - PubMed
    1. Imamura T, Fuji N, Suzuki A, Tamaki R, Saito M, Aniceto R, Galang H, Sombrero L, Lupisan S, Oshitani H. 2011. Enterovirus 68 among children with severe acute respiratory infection, the Philippines. Emerg Infect Dis 17:1430–1435. - PMC - PubMed
    1. Jacobson LM, Redd JT, Schneider E, Lu X, Chern SW, Oberste MS, Erdman DD, Fischer GE, Armstrong GL, Kodani M, Montoya J, Magri JM, Cheek JE. 2012. Outbreak of lower respiratory tract illness associated with human enterovirus 68 among American Indian children. Pediatr Infect Dis J 31:309–312. - PubMed
    1. Kaida A, Kubo H, Sekiguchi J, Kohdera U, Togawa M, Shiomi M, Nishigaki T, Iritani N. 2011. Enterovirus 68 in children with acute respiratory tract infections, Osaka, Japan. Emerg Infect Dis 17:1494–1497. - PMC - PubMed

Publication types

Substances

LinkOut - more resources