Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014:1202:103-10.
doi: 10.1007/7651_2013_37.

An assay to quantify chemotactic properties of degradation products from extracellular matrix

Brian M Sicari  1 , Li Zhang, Ricardo Londono, Stephen F Badylak
Affiliations

An assay to quantify chemotactic properties of degradation products from extracellular matrix

Brian M Sicari et al. Methods Mol Biol. 2014.

Abstract

The endogenous chemotaxis of cells toward sites of tissue injury and/or biomaterial implantation is an important component of the host response. Implanted biomaterials capable of recruiting host stem/progenitor cells to a site of interest may obviate challenges associated with cell transplantation. An assay for the identification and quantification of chemotaxis induced by surgically placed biologic scaffolds composed of extracellular matrix is described herein.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
An established assay for the identification of chemotaxis. Chemotaxis chamber used for the cell migration assay (a). Schematic representation of the chemotaxis assay (b). Outermost wells (solid circles) of the chamber are not used due to artifacts from edge evaporation (c). In a 48-well chamber up to ten substances can be tested (columns 2 through 11) in quadruplicate
See this image and copyright information in PMC

References

    1. Reing JE, Zhang L, Myers-Irvin J, Cordero KE, Freytes DO, Heber-Katz E, Bedelbaeva K, McIntosh D, Dewilde A, Braunhut SJ, Badylak SF. Degradation products of extracellular matrix affect cell migration and proliferation. Tissue Eng Part A. 2009;15(3):605–614. - PubMed
    1. Agrawal V, Siu BF, Chao H, Hirschi KK, Raborn E, Johnson SA, Tottey S, Hurley KB, Medberry CJ, Badylak SF. Partial Characterization of the Sox2+ Cell Population in an Adult Murine Model of Digit Amputation. Tissue Eng Part A. 2012;18(13):1454–1463. - PMC - PubMed
    1. Crisan M, Yap S, Casteilla L, Chen CW, Corselli M, Park TS, Andriolo G, Sun B, Zheng B, Zhang L, Norotte C, Teng PN, Traas J, Schugar R, Deasy BM, Badylak S, Buhring HJ, Giacobino JP, Lazzari L, Huard J, Peault B. A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008;3(3):301–313. - PubMed
    1. Agrawal V, Johnson SA, Reing J, Zhang L, Tottey S, Wang G, Hirschi KK, Braunhut S, Gudas LJ, Badylak SF. Epimorphic regeneration approach to tissue replacement in adult mammals. Proc Natl Acad Sci U S A. 2010;107(8):3351–3355. - PMC - PubMed
    1. Tottey S, Corselli M, Jeffries EM, Londono R, Peault B, Badylak SF. Extracellular matrix degradation products and low-oxygen conditions enhance the regenerative potential of perivascular stem cells. Tissue Eng Part A. 2011;17 (1–2):37–44. - PMC - PubMed

Substances

LinkOut - more resources