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. 2014:1195:33-41.
doi: 10.1007/7651_2013_42.

Growth and differentiation of HaCaT keratinocytes

Van G Wilson  1
Affiliations

Growth and differentiation of HaCaT keratinocytes

Van G Wilson. Methods Mol Biol. 2014.

Abstract

HaCaT cells are a spontaneously immortalized, human keratinocyte line that has been widely used for studies of skin biology and differentiation. Under typical culture conditions HaCaT cells have a partially to fully differentiated phenotype due to the high calcium content of both standard media and fetal bovine serum. This chapter describes low-calcium culture conditions for reverting HaCaT cells to the fully basal state followed by subsequent controlled differentiation using calcium induction.

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