Comparative Study to Assess the Effectiveness of Various Disinfecta- nts on two Microorganisms and the effect of same on Flexural Strength of Acrylic Denture Base Resin - An In Vitro Study

Abstract

Background: To evaluate and compare the effectiveness of various disinfectants on Candida albicans (C.albicans) and Staphylococcus aureus (Staph.aureus) inoculated on acrylic denture base resin and effect of disinfectants on flexural strength of denture base resin.

Materials & methods: A total of 130 acrylic denture base resin specimens were fabricated and processed according to manufacturer instructions. 82 sterile specimens were used for microbiological study. 2 specimens were cultured for organism growth to ensure sterility. 40 sterile specimens each were inoculated by immersing in Sabouraud & Nutrient broth containing microorganisms for 45 minutes each. Then the specimens were immersed in chlorhexidine, glutaraldehyde & distilled water (control) for 4 & 8 minutes. Then the specimens were neutralized. After neutralization the specimens were cultured onto Sabouraud's broth for C.albicans and Nutrient broth for Staph.aureus incubated for 72 h and observed for turbidity. At the end of 72 h subculture were made onto Sabourads dextrose agar media for C.albicans, Blood agar media for Staph.aureus and incubated for 48 h to observe growth. For flexural strength testing, 8 specimens each was immersed in the above mentioned disinfectants and distilled water for 8 & 16 minutes. Each of which was then subjected to 3 point flexural load in Lloyd's Universal testing machine. The peak load was recorded and flexural strength values were calculated.

Results: The microbiological study revealed that both disinfectants were equally effective at 4 minutes against C.albicans & Staph.aureus microorganisms. Flexural strength test revealed no significant difference between test and control groups.

Conclusion: Chlorhexdine and Glutaraldehyde disinfectans are equally effective against C.albicans and Staph.aureus microorganisms. Heat polymerized acrylic denture base resin did not demonstrate any significant change in flexural strength between control and test specimens. How to cite this article: Ganesh S, Gujjari A K, Shylesh K B S, Ravi M B, Sowmya S, Meenakshi S.Comparative Study to Assess the Effectiveness of Various Disinfectants on two Microorganisms and the effect of same on Flexural Strength of Acrylic Denture Base Resin - An In Vitro Study. J Int Oral Health 2013; 5(3):55-62.

Keywords: C.albicans; Chlorhexidine; Flexural strength.; Glutaraldehyde; Staph.aureus.