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. 1985 Dec;147(2):326-35.
doi: 10.1016/0042-6822(85)90135-7.

Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions

A D Hoffman, B Banapour, J A Levy

Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virions

A D Hoffman et al. Virology. 1985 Dec.

Abstract

The RNA-dependent DNA polymerase of the AIDS-associated retrovirus (ARV) gives highest activity with the synthetic template, poly(rA)oligo(dT) and prefers Mg2+ over Mn2+ as a divalent cation. It can use other template-primer combinations but with poly(rCm)oligo(dG), it prefers Mn2+ over Mg2+. Detection of ARV reverse transcriptase in culture fluids is substantially increased with an optimal KCl concentration and a special combination of EGTA and reducing agents. Our results indicate some distinguishing characteristics of the ARV reverse transcriptase and optimal conditions for its detection.

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