Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2013 Oct;25(5):613-8.
doi: 10.1016/j.coi.2013.09.010. Epub 2013 Oct 22.

Impact of new sequencing technologies on studies of the human B cell repertoire

Jessica A Finn  1 , James E Crowe Jr
Affiliations
Review

Impact of new sequencing technologies on studies of the human B cell repertoire

Jessica A Finn et al. Curr Opin Immunol. 2013 Oct.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Diversity in the antigen-combining site of the B cell receptor repertoire (and thus also in the corresponding secreted antibody repertoire) is mediated by three principal molecular mechanisms, illustrated in the three panels, left, middle, and right.
Figure 2
Figure 2
[A] Classical models of somatic hypermutation conceive of rapid generation of variants in the activated germinal center followed by a severe down-selection of number of variants, resulting in selection of only the clones with the most avidly binding B cell receptors for survival. [B] Newer repertoire studies using large-scale sequence analysis reveal that human B cell repertoires retain large number of variants with diverse numbers of point mutations within clones, even in the peripheral blood.
Figure 3
Figure 3
Types of repertoires: Private, from one donor, 2) Shared, sequences found in two or more donors, and 3) Global, the sequences in a population of subjects.
See this image and copyright information in PMC

References

    1. Lefranc M-P, Lefranc G. The Immunoglobulin FactsBook. Academic Press; 2001.
    1. Crotty S, Ahmed R. Immunological memory in humans. Sem Immunol. 2004;16:197–203. - PubMed
    1. Krause JC, Tsibane T, Tumpey TM, Huffman CJ, Briney BS, Smith SA, Basler CF, Crowe JE., Jr Epitope-specific human influenza antibody repertoires diversify by B cell intraclonal sequence divergence and interclonal convergence. J Immunol. 2011;187:3704–11. This is one of the first papers to reveal the persistence of highly related somatic variants of antibodies within clones responding to a virus, and also shows that genetically related but independent clones converge on common sequences during epitope-specific responses. - PMC - PubMed
    1. Lefranc MP, Giudicelli V, Ginestoux C, Jabado-Michaloud J, Folch G, Bellahcene F, Wu Y, Gemrot E, Brochet X, Lane J, et al. IMGT, the international ImMunoGeneTics information system. Nucl Acids Res. 2009;37:D1006–D1012. - PMC - PubMed
    1. Munshaw S, Kepler TB. SoDA2: A hidden Markov model approach for identification of immunoglobulin rearrangements. Bioinformatics. 2010;26:867–72. - PMC - PubMed

Publication types

LinkOut - more resources