doi: 10.1007/978-1-62703-718-1_4.
Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture
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- PMID: 24166367
- DOI: 10.1007/978-1-62703-718-1_4
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Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture
Methods Mol Biol.
2014.
Abstract
Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter.
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