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. 2014:1098:47-55.
doi: 10.1007/978-1-62703-718-1_4.

Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture

M Hannah Degeling  1 , M Sarah S Bovenberg, Marie Tannous, Bakhos A Tannous
Affiliations

Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture

M Hannah Degeling et al. Methods Mol Biol. 2014.

Abstract

Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter.

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