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. 2013 Sep;9(3):153-61.

Effects of Chinese Herbal Compound "Xuemai Ning"on Rabbit Atherosclerosis Model and Expression of ABCA1

Min Chen  1
Affiliations

Effects of Chinese Herbal Compound "Xuemai Ning"on Rabbit Atherosclerosis Model and Expression of ABCA1

Min Chen. Int J Biomed Sci. 2013 Sep.

Abstract

Objective: To observe the lipid and the pathological changes of carotid artery smooth muscle cells in atherosclerotic rabbits, verification of Chinese herbal compound which has improve blood lipid and anti atherosclerosis effects, focus on ABCA1 as the key receptor which participated in reverse cholesterol transport, to study the mechanism of Chinese herbal compound (Xuemai Ning).

Materials and methods: 30 rabbits were randomly divided into blank group, model group and Chinese herbal compound (Xuemai Ning) group, The model group and the Xuemai Ning group with high fat diet and injection of vitamin D3, causing atherosclerosis model 4 weeks after the intervention of traditional Chinese medicine group, In the 4th week after Xuemai Ning group received the intervention of Chinese herbal compound. Blood lipid, the carotid artery pathological changes and expression of ABCA1 gene and protein in peritoneal macrophage surface were detected after 8 weeks.

Results: The carotid artery atherosclerotic plaque formation of the model group was obvious, the carotid atherosclerotic changes of the Xuemai Ning group rabbit significantly lighter than the model group. The serum lipid of model group and Xuemai Ning group were higher than that of the blank group; and the traditional Chinese medicine can up the expression of ABCA1 protein, higher than those in the model group. Expression of macrophage ABCA1 in model group was significantly up regulated at protein level higher than the blank group; and the traditional Chinese medicine can up regulate the expression of ABCA1 protein, higher than those in the model group. Expression of ABCA1 mRNA was significantly up regulated in model group, ABCA1 mRNA of Xuemai Ning group raised more significantly.

Conclusion: Xuemai Ning can reduce triglyceride, total cholesterol and low density lipoprotein of hyperlipidemia model in rabbits serum, increase high density lipoprotein, remove foam cells in atherosclerotic cells, improve pathological of AS and up-regulate ABCA1 gene and protein so as to effectively inhibit atherosclerotic disease.

Keywords: ATP binding cassette transporter A1 (ABCA1); Atherosclerosis; Blood lipid; Chinese herbal medicine; Reverse Cholesterol Transport (RCT).

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Figures

Figure 1
Figure 1
Pathological changes of All rabbits carotid artery (HE ×100 times).
Figure 3
Figure 3
Determination results of Rabbit blood lipid content.
Figure 2
Figure 2
In rabbit peritoneal macrophage lipid-loaded (oil red o stain, ×400 times).
Figure 4
Figure 4
The results of the amount of lipid loaded macrophages.
Figure 5
Figure 5
The expression level of ABCA1 protein in peritoneal macrophages.
Figure 6
Figure 6
In rabbit peritoneal macrophage cell surface expression of ABCA1 protein (DAB color rendering, ×400 times).
Figure 7
Figure 7
Rabbit abdominal macrophage ABCA1 mRNA expression results.
Figure 8
Figure 8
Rabbit abdominal macrophage ABCA1 mRNA expression results. 187 bp ABCA1 gene amplification products; 410 bp beta-actin gene amplification products. M, Marker; 1. blank group; 2. model group; 3. chinese herbal group.
See this image and copyright information in PMC

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