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Comment
. 2013 Oct 29:2:e01561.
doi: 10.7554/eLife.01561.

Proteins pinpoint double strand breaks

Michael M Cox  1
Affiliations
Comment

Proteins pinpoint double strand breaks

Michael M Cox. Elife. .

Abstract

Combining green fluorescent protein with a protein that only binds to double strand breaks in DNA allows these breaks-which are an important form of DNA damage-to be detected with high efficiency in living bacteria.

Keywords: DNA double-strand breaks; E. coli; GFP; Human; Mouse; endogenous DNA damage; fluorescent-protein fusions; spontaneous DNA breaks; synthetic biology.

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Conflict of interest statement

Competing interests:The author declares that no competing interests exist.

Figures

Figure 1.
Figure 1.. How double strand breaks occur during replication.
Many DNA repair processes generate transient single strand breaks in chromosomes. If a replication fork encounters such a break before it is repaired (top and middle), one arm of the replication fork separates to create a double strand break (bottom).
See this image and copyright information in PMC

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