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. 1984 Dec;9(1):75-82.
doi: 10.1007/BF00396207.

Mitochondrial DNA of Chlamydomonas reinhardtii: the DNA sequence of a region showing homology with mammalian URF2

E Pratje  1 , S Schnierer, B Dujon
Affiliations

Mitochondrial DNA of Chlamydomonas reinhardtii: the DNA sequence of a region showing homology with mammalian URF2

E Pratje et al. Curr Genet. 1984 Dec.

Abstract

A 1.27 kb DNA fragment of the 15 kb DNA of Chlamydomonas reinhardtii has been cloned and sequenced. A 906 bp long open reading frame was found showing homology with the URF2 genes of mammals and insects. This homology is functional evidence for Chlamydomonas reinhardtii 15 kb DNA representing indeed mitochondrial DNA. This is the first report of an URF2 gene in mitochondria of a photosynthetic organism. The absence of a TGA codon within the gene suggests that it is used as stop codon like in higher plants and not as tryptophan like in animal and fungal mitochondria.

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