Ionic-liquid-assisted microwave distillation coupled with headspace single-drop microextraction followed by GC-MS for the rapid analysis of essential oil in Dryopteris fragrans

Abstract

A rapid, green and effective miniaturized sample preparation technique, ionic-liquid-assisted microwave distillation coupled with headspace single-drop microextraction was developed for the extraction of essential oil from dried Dryopteris fragrans. 1-Ethyl-3-methylimidazolium acetate was the optimal ionic liquid as the destruction agent of plant cell walls and microwave absorption was medium. n-Heptadecane (2.0 μL) was adopted as the suspended microdrop solvent in the headspace for the extraction and concentration of essential oil. The optimal parameters of the proposed method were an irradiation power of 300 W, sample mass of 0.9 g, mass ratio of ionic liquids to sample of 2.8, extraction temperature of 79°C, and extraction time of 3.6 min. In comparison to the previous reports, the proposed technique could equally monitor all the essential oil components with no significant differences in a simple way, which was more rapid and required a much lower amount of sample.

Keywords: Dryopteris fragrans; Essential oils; Ionic liquids; Microextraction; Microwave distillation.