Saliva metabolomics by NMR for the evaluation of sport performance

Abstract

The paper reports preliminary results of a study in order to verify that saliva is a bio-fluid sensitive to metabolite variations due to stress and fatigue in soccer athletes, and possibly, to identify potential markers of test of performance. Saliva samples of fourteen professional soccer players were collected before and after the stressful physical activity of the level 1 Yo-Yo intermittent recovery test and, also, physiological parameters were evaluated. The NMR spectra of saliva offer a metabolites profiling which was analyzed by Principal Component Analysis as a blind test. The results of NMR pre and post test shows that it was possible to cluster the best and the worst performing athletes and that the role of the actual player may be diagnosed by a different cluster of metabolites profile. Thus saliva can be considered a biofluid metabolically sensitive to the induced physical stress and, in the future, deeper investigated to monitor the performances in athletes.

Keywords: 1D; IR; Intermittent recovery Yo-Yo test; NMR; NMR Metabolomics; PCA; Physical exercise; Principal Component Analysis; Salivary metabolites; intermittent recovery; mono-dimensional; nuclear magnetic resonance.