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. 1984 May;8(4):271-6.
doi: 10.1007/BF00419724.

Isolation of a selective marker from Cephalosporium acremonium by complementation of an auxotrophic mutant of E. coli

E Friedlin  1 , J Nüesch
Affiliations

Isolation of a selective marker from Cephalosporium acremonium by complementation of an auxotrophic mutant of E. coli

E Friedlin et al. Curr Genet. 1984 May.

Abstract

A Cephalosoporium acremonium gene library has been constructed using cosmid vector pYcl. After transformation of E. coli leuB strain JA221 with cosmids isolated from this gene library a cosmid (pCeleuB43) was isolated which complements the leucine auxotrophy of JA221. The same cosmid is also able to complement a leu2 strain of S. cerevisiae. The leuB and leu2 genes both code for β-isopropylmalate dehydrogenase. The complementation of a leuB E. coli strain and a leu2 S. cerevisiae strain strongly suggests that the analogous gene from C. acremonium has been isolated.

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