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. 1996 Aug;15(11):855-8.
doi: 10.1007/BF00233156.

Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica

L Garg  1 , N N Bhandari, V Rani, S S Bhojwani
Affiliations

Somatic embryogenesis and regeneration of triploid plants in endosperm cultures of Acacia nilotica

L Garg et al. Plant Cell Rep. 1996 Aug.

Abstract

Immature endosperm of Acacia nilotica formed a nodular callus on MS medium supplemented with 2,4-D, BAP and CH. In the third passage on this medium, in the dark, the callus differentiated somatic embryos. The embryos germinated on MS only after 15 d pre-treatment on modified MS medium in which major salts were replaced by those of major salts of B5 medium and supplemented with glutamine, CH and CW. Triploid nature of the somatic embryos was confirmed by Feulgen cytophotometry.

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References

    1. Theor Appl Genet. 1990 Dec;80(6):785-90 - PubMed
    1. Exp Cell Res. 1968 Apr;50(1):151-8 - PubMed