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. 1986 Feb;72(2):129-32.
doi: 10.1007/BF00283930.

Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. I. Demonstration of replication patterns

W Vogel, M Autenrieth, G Speit

Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. I. Demonstration of replication patterns

W Vogel et al. Hum Genet. 1986 Feb.

Abstract

We have shown that a commercially available antibody allows the demonstration of bromodeoxyuridine (BrdU) incorporation into mammalian chromosomes by the typical immunologic staining techniques. The replication patterns obtained are comparable to those after BrdU-incorporation and FPG-staining, though with reversed staining specificity. The quality of the patterns equals that of commonly used differential staining but the sensitivity of BrdU-detection is superior. Thus the new technique may be very promising for all cytogenetic studies concerning any kind of chromosome replication.

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References

    1. Dev Biol Stand. 1987;66:91-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3395-9 - PubMed
    1. Cytogenet Cell Genet. 1981;31(1):5-23 - PubMed
    1. Hum Genet. 1984;67(3):264-9 - PubMed
    1. Chromosoma. 1978 Jun 28;67(2):193-9 - PubMed

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