Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1986 Jan;57(1):55-60.

Different pathways of human T-cell activation revealed by PHA-P and PHA-M

K O'Flynn, M Russul-Saib, I Ando, D L Wallace, P C Beverley, A W Boylston, D C Linch
Comparative Study

Different pathways of human T-cell activation revealed by PHA-P and PHA-M

K O'Flynn et al. Immunology. 1986 Jan.

Abstract

Antigen-specific T-cell activation is mediated via the CD3-Ti (antigen receptor) complex, and monoclonal antibodies to both CD3 and Ti cause a rapid rise in intracellular Ca2+. This calcium mobilization is not inhibited by monoclonal antibodies to CD2. The rise in calcium mobilization induced by purified PHA (PHA-P) does not occur in a cell line which lacks CD2 expression, and can be blocked in other T cells by anti-CD2 antibodies. A combination of monoclonal antibodies to different epitopes of CD2 causes calcium mobilization and mitogenesis. Reagent grade PHA (PHA-M) induces calcium moblization in cells that lack CD2, and its effects in other T cells cannot be blocked by anti-CD2 antibodies. The effects of PHA-P and PHA-M are thus mediated predominantly through different activation pathways.

PubMed Disclaimer

References

    1. J Natl Cancer Inst. 1978 Jun;60(6):1269-77 - PubMed
    1. J Biol Chem. 1981 Jun 25;256(12):6148-54 - PubMed
    1. Eur J Immunol. 1981 Apr;11(4):329-34 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7489-93 - PubMed
    1. J Immunol. 1983 Jul;131(1):180-5 - PubMed

Publication types

LinkOut - more resources