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. 2013:533:225-33.
doi: 10.1016/B978-0-12-420067-8.00015-5.

Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry

Kirstie Canene-Adams  1
Affiliations

Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry

Kirstie Canene-Adams. Methods Enzymol. 2013.

Abstract

The purpose of this protocol is to take any biopsy or whole organ tissue from animals or human, formalin-fix the specimen to preserve the current state of the tissue, and embed it into a paraffin block and for future immunohistochemistry experiments (If you intend to fix cells, check the alternative protocols: Preparation of Cells for Microscopy using Cytospin, Preparation of Cells for Microscopy using Chamber Slides and Coverslips, or Preparation of Cells for Microscopy using 'Cell Blocks').

Keywords: Fixation of tissue; Formalin-fixed paraffin-embedded tissue (FFPET); Immunohistochemistry; Tissue process and embedded; Tissue section.

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