Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Apr;14(7):403-6.
doi: 10.1007/BF00234043.

Green fluorescent protein: an in vivo reporter of plant gene expression

R P Niedz  1 , M R Sussman, J S Satterlee
Affiliations

Green fluorescent protein: an in vivo reporter of plant gene expression

R P Niedz et al. Plant Cell Rep. 1995 Apr.

Abstract

Protoplasts were isolated from H89, an embryogenic sweet orange (Citrus sinensis (L.) Osbeck cv. Hamlin) suspension culture, and electroporated with p35S-GFP, a plasmid carrying the gene for the green fluorescent protein (GFP) from the bioluminescent jellyfish Aequorea victoria. p35S-GFP was constructed by replacing the GUS coding sequence of pBI221 with a functional GFP gene, thereby placing the GFP gene under the control of the CaMV 35S promoter. Protoplasts were viewed by incident-light fluorescence microscopy twentyfour h after electroporation. 20-60% of the protoplasts emitted an intense green light when illuminated with blue (450-490 nm) light.

PubMed Disclaimer

References

    1. Gene. 1992 Feb 15;111(2):229-33 - PubMed
    1. Plant Cell Rep. 1988 Jun;7(4):225-8 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Biotechniques. 1989 Nov-Dec;7(10):1124-31 - PubMed
    1. Nature. 1994 Jun 2;369(6479):400-03 - PubMed

LinkOut - more resources