Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Apr;88(1):75-80.
doi: 10.1007/BF00222397.

Production of somatic hybrids between Daucus carota L. and Nicotiana tabacum

H Kisaka  1 , T Kameya
Affiliations

Production of somatic hybrids between Daucus carota L. and Nicotiana tabacum

H Kisaka et al. Theor Appl Genet. 1994 Apr.

Abstract

Protoplasts of a kanamycin-resistant (KR, nuclear genome), streptomycin-resistant (SR, chloroplast genome) and chlorophyll-deficient (A1, nuclear genome) Nicotiana tabacum (KR-SA) cell suspension cultures or X-ray-irradiated mesophyll protoplasts of kanamycin- and streptomycin-resistant green plants (KR-SR) were fused with protoplasts of a cytoplasmic male-sterile (CMS) Daucus carota L. cell suspension cultures by electrofusion. Somatic hybrid plants were selected for kanamycin resistance and the ability to produce chlorophyll. Most of the regenerated plants had a normal D. carota morphology. Callus induced from these plants possessed 23-32 chromosomes, a number lower than the combined chromosome number (66) of the parents, and were resistant to kanamycin, but they segregated for streptomycin resistance, which indicated that N. tabacum chloroplasts had been eliminated. Genomic DNA from several regenerated plants was analyzed by Southern hybridization for the presence of the neomycin phosphotransferase gene (NPTII); all of the plants analyzed were found to contain this gene. Mitochondrial (mt) DNA was analyzed by Southern hybridization of restriction endonuclease digests of mtDNA with two DNA probes, PKT5 and coxII. The results showed that the two plants analyzed possessed the mitochondria of D. carota. These results demonstrate that the regenerated plants are interfamilial somatic hybrids.

PubMed Disclaimer

References

    1. Theor Appl Genet. 1989 May;77(5):641-4 - PubMed
    1. Theor Appl Genet. 1989 May;77(5):651-6 - PubMed
    1. Jpn J Genet. 1991 Dec;66(6):719-24 - PubMed
    1. Theor Appl Genet. 1990 Nov;80(5):588-92 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8434-8 - PubMed

LinkOut - more resources