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Comparative Study
. 2013 Oct 28;19(40):6883-7.
doi: 10.3748/wjg.v19.i40.6883.

Expression and significance of cyclooxygenase-2 mRNA in benign and malignant ascites

Jing Lu  1 , Xiao-Feng Li, Li-Xia Kong, Lin Ma, Su-Huan Liao, Chang-You Jiang
Affiliations
Comparative Study

Expression and significance of cyclooxygenase-2 mRNA in benign and malignant ascites

Jing Lu et al. World J Gastroenterol. .

Abstract

Aim: To investigate the mRNA expression of cyclooxygensae-2 (COX-2) in benign and malignant ascites, and to explore the difference in COX-2 mRNA expression among different diseases.

Methods: A total of 36 samples were collected from the Fifth Affiliated Hospital of Sun Yat-Sen University and divided into two experimental groups: benign ascites (n = 21) and malignant ascites (n = 15). Benign ascites included cirrhotic ascites (n = 10) and tuberculous ascites (n = 5). Malignant ascites included oophoroma (n = 7), cancer of colon (n = 5), cancer of the liver (n = 6), gastric cancer (n = 2), and bladder carcinoma (n = 1). The mRNA expression of COX-2 in ascites was examined with reverse transcriptase polymerase chain reaction (RT-PCR) technology, and the positive rate of COX-2 mRNA was compared between different diseases.

Results: The positive rate of COX-2 mRNA in malignant ascites was 42.9% (9/21), which was significantly higher than in benign ascites, 6.7% (1/15), difference being significant between these two groups (χ(2) = 4.051, P = 0.044). The proportion of the positive rate in the malignant ascites was as follows: ovarian cancers 57.1% (4/7), colon cancer 40.0% (2/5), liver cancer 33.3% (2/6), gastric cancer 50.0% (1/2), and bladder cancer 0.00% (0/1). However, there was no significant difference in COX-2 mRNA expression among various tumors with malignant ascites (χ(2) = 1.614, P = 0.806). Among the benign ascites, COX-2 mRNA levels were different between the tuberculous ascites (0/5) and cirrhotic ascites (1/10), but there was no significant difference (P = 1.000).

Conclusion: COX-2 mRNA, detected by RT-PCR, is useful in the differential diagnosis of benign and malignant ascites, which also has potential value in the clinical diagnosis of tumors.

Keywords: Ascites; Cyclooxygensae-2 mRNA; Malignant tumor; Reverse transcriptase polymerase chain reaction.

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References

    1. Metzgeroth G, Kuhn C, Schultheis B, Hehlmann R, Hastka J. Diagnostic accuracy of cytology and immunocytology in carcinomatous effusions. Cytopathology. 2008;19:205–211. - PubMed
    1. Gu H, Deng XY, Yan L, Zhang GY. The diagnosis potential of the ratio for the differentiation between benign and malignant ascites by the combined measurement of ascites and serum tumor markers as well as the F/S ratio. Zhongguo Xiandai Yixue Zazhi. 2011;14:1595–1599.
    1. Hill R, Li Y, Tran LM, Dry S, Calvopina JH, Garcia A, Kim C, Wang Y, Donahue TR, Herschman HR, et al. Cell intrinsic role of COX-2 in pancreatic cancer development. Mol Cancer Ther. 2012;11:2127–2137. - PMC - PubMed
    1. Jiang H, Wang J, Zhao W. Cox-2 in non-small cell lung cancer: a meta-analysis. Clin Chim Acta. 2013;419:26–32. - PubMed
    1. Lurje G, Vallbohmer D, Collet PH, Xi H, Baldus SE, Brabender J, Metzger R, Heitmann M, Neiss S, Drebber U, et al. COX-2 mRNA expression is significantly increased in acid-exposed compared to nonexposed squamous epithelium in gastroesophageal reflux disease. J Gastrointest Surg. 2007;11:1105–1111. - PubMed

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