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. 1986 Jan 14;134(1):146-51.
doi: 10.1016/0006-291x(86)90539-5.

Selection of a rat glutamine synthetase cDNA clone

D M Burns, B Bhandari, J M Short, P G Sanders, R H Wilson, R E Miller

Selection of a rat glutamine synthetase cDNA clone

D M Burns et al. Biochem Biophys Res Commun. .

Abstract

We have selected a glutamine synthetase clone (pGSRK-1) from a rat kidney cDNA library. A partial restriction map has been constructed for the 1.65 kilobase pair (kbp) glutamine synthetase cDNA. Northern hybridization analysis indicates that 1) GS-specific RNA increases many-fold during adipocyte differentiation and 2) dexamethasone increases and insulin decreases GS-specific RNA in 3T3-L1 adipocytes.

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