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. 1980 Oct;2(2):97-101.
doi: 10.1007/BF00420620.

Isolation and characterization of mitochondrial DNA from the alkane yeast Saccharomycopsis lipolytica

U Kück  1 , U Stahl, A Lhermitte, K Esser
Affiliations

Isolation and characterization of mitochondrial DNA from the alkane yeast Saccharomycopsis lipolytica

U Kück et al. Curr Genet. 1980 Oct.

Abstract

Mitochondrial (mt) DNA of the alkane yeast, Saccharomycopsis lipolytica, was isolated. Its buoyant density in CsCl was found to be of 1.687 g/cm(3), indicating a GC content of 27.5% and its melting point Tm = 79.5 °C, indicating a GC content of 24.9%. The corresponding values for nuclear (n) DNA, are 1.709 g/cm(3) (GC: 49.5%) and Tm = 90.5 (GC: 51.7%) respectively. Electron microscopy revealed that mtDNA has a circular structure with a contour length of about 14.5 µm corresponding to 45.5 kb per molecule. The size estimated from restriction analyses performed with 7 endonucleases was 48.35 kb/molecule. A restriction map was constructed, using the cleavage data of 4 endonucleases.

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