Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jan;14(4):253-6.
doi: 10.1007/BF00233644.

Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.)

M Salmenkallio-Marttila  1 , V Kauppinen
Affiliations

Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.)

M Salmenkallio-Marttila et al. Plant Cell Rep. 1995 Jan.

Abstract

Cultures of isolated microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar of malting barley) were used for isolation of protoplasts. The protoplasts were cultured embedded in agarose. The plating efficiency varied from 0.002% to 0.015%. Several hundred green plants were regenerated from the cultures. Plantlets regenerated from protoplasts were potted in soil within 4-5 months of collecting the spikes for microspore culture and the first plants are now setting seed.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Science. 1988 Apr 8;240(4849):204-7 - PubMed
    1. Planta. 1988 Jul;175(1):71-81 - PubMed
    1. Theor Appl Genet. 1991 Apr;81(4):437-44 - PubMed
    1. Plant Cell Rep. 1991 May;10(1):1-6 - PubMed
    1. Theor Appl Genet. 1988 Dec;76(6):835-40 - PubMed