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. 2013 Nov;69(Pt 11):1246-8.
doi: 10.1107/S1744309113026018. Epub 2013 Oct 19.

Cloning, purification, crystallization and preliminary X-ray studies of the putative transcriptional regulator SPD0280 from Streptococcus pneumoniae D39

Shaocheng Zhang  1 , Xun Min, Zhen Guo, Hongpeng Zhang, Ailong Huang, Yibing Yin, Deqiang Wang
Affiliations

Cloning, purification, crystallization and preliminary X-ray studies of the putative transcriptional regulator SPD0280 from Streptococcus pneumoniae D39

Shaocheng Zhang et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov.

Abstract

Streptococcus pneumoniae SPD0280 is a hypothetical protein that has been putatively identified as a transcriptional regulator. However, it has very low sequence identity to other well characterized transcriptional regulators. Determination of the three-dimensional structure may provide information for the characterization of proteins; therefore, it was decided to use X-ray diffraction analysis to learn more about this protein. Here, the expression, purification, crystallization and preliminary crystallographic analysis of SPD0280 from S. pneumoniae are reported. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 45.886, b = 66.785, c = 150.050 Å, and diffracted to a resolution of 2.5 Å. The crystals are likely to contain one molecule in the asymmetric unit, with a VM value of 2.06 Å(3) Da(-1).

Keywords: SPD0280; Streptococcus pneumoniae.

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Figures

Figure 1
Figure 1
Crystals of SPD0280 from S. pneumoniae D39. The dimensions of the crystals are 0.02 × 0.02 × 0.5 mm.
Figure 2
Figure 2
X-ray diffraction pattern of a crystal of SPD0280 from S. pneumoniae D39 obtained at the Shanghai Synchrotron Radiation Facility (SSRF). The circle indicates 3.5 Å resolution.
See this image and copyright information in PMC

References

    1. Anbalagan, S., Dmitriev, A., McShan, W. M., Dunman, P. M. & Chaussee, M. S. (2012). J. Bacteriol. 194, 3961–3971. - PMC - PubMed
    1. Berkley, J. A., Lowe, B. S., Mwangi, I., Williams, T., Bauni, E., Mwarumba, S., Ngetsa, C., Slack, M. P., Njenga, S., Hart, C. A., Maitland, K., English, M., Marsh, K. & Scott, J. A. (2005). N. Engl. J. Med. 352, 39–47. - PubMed
    1. Blomberg, C., Dagerhamn, J., Dahlberg, S., Browall, S., Fernebro, J., Albiger, B., Morfeldt, E., Normark, S. & Henriques-Normark, B. (2009). J. Infect. Dis. 199, 1032–1042. - PubMed
    1. Graille, M., Zhou, C.-Z., Receveur-Bréchot, V., Collinet, B., Declerck, N. & van Tilbeurgh, H. (2005). J. Biol. Chem. 280, 14780–14789. - PubMed
    1. Gupta, R., Bhatty, M., Swiatlo, E. & Nanduri, B. (2013). PLoS One, 8, e55157. - PMC - PubMed

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