doi: 10.1007/BF00232939.
Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement
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- PMID: 24196247
- DOI: 10.1007/BF00232939
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Easy determination of ploidy level in Arabidopsis thaliana plants by means of pollen size measurement
Plant Cell Rep.
1994 Aug.
Abstract
Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.
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