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. 1988 Jul;16(1):17-29.
doi: 10.1007/BF02097402.

Microbial communities in the saturated groundwater environment I: Methods of isolation and characterization of heterotrophic bacteria

J Kölbel-Boelke  1 , B Tienken, A Nehrkorn
Affiliations

Microbial communities in the saturated groundwater environment I: Methods of isolation and characterization of heterotrophic bacteria

J Kölbel-Boelke et al. Microb Ecol. 1988 Jul.

Abstract

In this paper we present a method of isolation and morphological and physiological characterization of groundwater bacteria based on numerical taxonomy and cluster analysis, and using a miniaturized test system (microtiter plates). Bacteria were isolated randomly on P-agar, and each strain was characterized in regard to 155 features. The media for biochemical differentiation are listed as well as methods of morphological discrimination. 246 strains of heterotrophic and oligotrophic bacteria, isolated from five water samples from different depths of the saturated groundwater area, were used for optimizing media and test reactions.

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