Composition of the microflora of witloof chicory seeds

Abstract

The bacterial microflora of nine varieties of witloof chicory (Cichorium intybus L. var.foliosum Hegi) seeds was studied. The 184 isolates were characterized by protein profiles determined by SDS-protein polyacrylamide gel electrophoresis of the total cell proteins. Isolates with identical protein profiles were grouped into one fingerprint type. Sixty-seven fingerprint types were distinguished. Two quantitatively major fingerprint types,Erwinia herbicola and an arthrobacter, represented 52% of the total number of isolates and were found on different chicory varieties. The latter organism was inhibited at seed germination. Other isolates, i.e.,Xanthomonas maltophilia, Pseudomonas paucimobilis, Agrobacterium radiobacter, Pseudomonas syringae, and a fluorescentPseudomonas, were only occasionally found. A minority were gram-positive isolates, i.e.,Bacillus sp.,Streptomyces sp., and coryneforms. In vitro activity of the isolates was tested against five fungi. Isolates with strong antifungal activity were found amongErwinia herbicola andBacillus sp.