Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1993 Aug;12(10):537-40.
doi: 10.1007/BF00233055.

Regeneration of protoplast-derived green plants of Kentucky blue grass (Poa pratensis L.)

K A Nielsen  1 , E Larsen, E Knudsen
Affiliations

Regeneration of protoplast-derived green plants of Kentucky blue grass (Poa pratensis L.)

K A Nielsen et al. Plant Cell Rep. 1993 Aug.

Abstract

Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three different suspension lines varied from 0.004% in the lowest up to 1.5% in the highest responding line, using agarose-bedding in nutrition medium devoid of nurse or feeder cultures. Green plants germinated from polyembryos, which developed from 0.4-2.7% of the protoplast-derived microcolonies. A total of 127 plants were successfully transferred to soil.

PubMed Disclaimer

References

    1. Mol Gen Genet. 1991 Dec;231(1):1-6 - PubMed
    1. Planta. 1988 Jul;175(1):71-81 - PubMed
    1. Plant Cell Rep. 1989 May;8(5):282-4 - PubMed
    1. Plant Cell Rep. 1991 May;10(1):1-6 - PubMed
    1. Plant Cell Rep. 1988 Dec;7(7):469-72 - PubMed