A 1,4-β-D-glucan-synthase system from Dictyostelium discoideum

Abstract

Particulate membrane preparations have been isolated from culminating Dictyostelium discoideum cells. The preparations incorporated glucose from uridine 5'-diphosphate-glucose into a glucose polymer or polymers. These have been shown to be homopolymers of β-linked glucose. A high percentage (78% by methylation analysis) of the linkages formed are 1,4-linkages and a lower percentage (12%) are 1,3-linkages. The glucan-synthase complex present in the particulate membrane preparation has an apparent Km of 0.28 mM and a Vmax of 1.59 nmol·min(-1)·(mg protein)(-1). The enzyme system is dependent upon Mg(2+) and cellobiose for maximal activity, but is inhibited by millimolar levels of Ca(2+). Particulate membrane preparations were made from cells at various times during a synchronous developmental time course and demonstrated that the glucan-synthase activity appeared at the tight-aggregate stage of development.