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. 1993 Jan;12(2):107-9.
doi: 10.1007/BF00241944.

Use of RAPD markers to screen somatic hybrids between Solanum tuberosum and S. brevidens

Y S Xu  1 , M S Clark, E Pehu
Affiliations

Use of RAPD markers to screen somatic hybrids between Solanum tuberosum and S. brevidens

Y S Xu et al. Plant Cell Rep. 1993 Jan.

Abstract

The identification of somatic hybrids between Solanum tuberosum and S. brevidens can be carried out using polymerase chain reaction (PCR) and arbitrary 10-mer primers to generate random amplified polymorphic DNA (RAPD) markers. Five commercial primers have been tested. Each primer directed the amplification of a genome-specific "fingerprint" for the fusion parents and S. brevidens. The size of the amplified DNA fragments ranged from 100 to 1800 base pairs. The somatic hybrids showed a combination of the parental banding profiles with four of the five primers surveyed, whereas regenerants from one of the parents had the same or a similar banding pattern to that of the parent. Thus RAPD markers provide a quick, simple and preliminary screening method for putative somatic hybrids.

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