Characterization and organization of gene families at the Gli-1 loci of bread and durum wheats by restriction fragment analysis

Abstract

Probes related to γ-gliadins and to the LMW subunits of glutenin were used to determine the complexity of the Gli-1 loci, by RFLP analysis of euploid and aneuploid lines of bread wheat cv Chinese Spring and durum wheat cv Langdon. The two probes hybridised to separate sets of fragments derived from chromosomes 1 A, 1 B and 1D. The fragments related to the LMW subunit probe had a total copy number in HindIII digests of about 35 in Chinese Spring and 17 in Langdon, with more fragments derived from chromosomes 1D. The fragments hybridising to the γ-gliadin probe could be divided into two classes, based on whether they hybridised to the whole probe at high stringency or to the 3' nonrepetitive region at moderate stringency. The fragments that failed to hybridise under these conditions were considered to be related to ω-gliadins. The fragments related to γ - and co-gliadins had total copy numbers of about 39 and 16, respectively, in HindIII digests of Chinese Spring, and about 24 and 12, respectively, in Langdon.