Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1996 Dec;7(12):1270-2.
doi: 10.1016/S1044-0305(96)00152-3.

Long-lived metallized tips for nanoliter electrospray mass spectrometry

G A Valaskovic  1 , F W McLafferty
Affiliations

Long-lived metallized tips for nanoliter electrospray mass spectrometry

G A Valaskovic et al. J Am Soc Mass Spectrom. 1996 Dec.

Abstract

Sheathless electrospray at nL/min flow rates combined with Fourier-transform mass spectrometry has made possible high resolving power (>50,000) mass spectra of subattomole samples of >8 kDa proteins separated by capillary electrophoresis (Valaskovic, G. A.; Kelleher, N. L.; McLafferty, F. W. Science, 1996, 273, 1199-1202). However, for this new method the mechanical stability of the thin (35 to 100 nm) gold film electrodes has limited tip lifetime to 15 to 30 min. A technique for SiO x coating of the gold is described that provides a steady ion current (±10 pA) for 1 to 2 h, even with arcs or interruptions of the electrospray voltage.

PubMed Disclaimer

References

    1. Anal Chem. 1995 Oct 15;67(20):3802-5 - PubMed
    1. Science. 1996 Aug 30;273(5279):1199-202 - PubMed
    1. Rapid Commun Mass Spectrom. 1993 Aug;7(8):738-43 - PubMed
    1. Anal Chem. 1994 Feb 1;66(3):406-14 - PubMed
    1. Science. 1989 Oct 6;246(4926):64-71 - PubMed

LinkOut - more resources