Tula hantavirus infection in immunocompromised host, Czech Republic

Abstract

We report molecular evidence of Tula hantavirus as an etiologic agent of pulmonary-renal syndrome in an immunocompromised patient. Acute hantavirus infection was confirmed by using serologic and molecular methods. Sequencing revealed Tula virus genome RNA in the patient's blood. This case shows that Tula virus can cause serious disease in humans.

Keywords: Tula virus; hantavirus; hantavirus pulmonary syndrome; hemorrhagic fever with renal syndrome; immunocompromised host; viruses.

Figure 1

Phylogenetic tree (neighbor-joining analysis with maximum composite likelihood method) of Tula virus on the basis of large segment partial sequences (nt 2957–3337), Ostrava, Czech Republic, October 2012 GenBank accesion numbers: Haantaan virus (NC_005222), Puumala virus (Z66548), Prospect Hill virus (EF646763), 09/1905/Magr (HQ728460), 08/712/Arv (HQ728453), 09/2155/Arv (HQ728456), 08/525/Marv (HQ728461), 152/Arv (HQ728459), 78/Marv (HQ728464), 20/Marv (HQ728462), 109/Arv (HQ728457), 127/Arv (HQ728458), 09/1026/Arv (HQ728455), Moravia/5302v (AJ005637), JiTr/Opava /12 (KC522413), Hodos/Ma99/99 (FJ495101), Sred ob Dravi/Ms51/97 (FJ495102), Griblje/Ma57/01 (FJ495099), Sestrze/Mag98/02 (FJ495100). Bootstrap values ≥70%, calculated from 1,000 replicates, are shown at the tree branches. Arrow indicates strain isolated in this study. The tree is drawn to scale. The scale bar indicates an evolutionary distance of 0.05 substitutions per position in the sequence.

Figure 2

Phylogenetic tree (neighbor-joining analysis with maximum composite likelihood method) of Tula virus on the basis of small segment partial sequences (nt 428–758), Ostrava, Czech Republic, October 2012 GenBank accession numbers: Haantaan virus (NC_005218), Puumala virus (NC_005224), Prospect Hill virus (Z49098), Isla Vista virus (U19302), Karatal322 (AM945877), Taldykorgan343 (AM945879), Karatal340 (AM945878), Omsk23 (AF442621), Tula76 (Z30941), Tula53 (Z30942), Tula175 (Z30943), Lodz-1 (AF063892), Lodz-2 (AF063897), Cottbus/D63/98 (AF289821), Cottbus/D5/98 (AF289819), Cacak/Serbia (AF017659), Kosice/667 (Y13980), Kosice/144 (Y13979), Waldnaab/g20-s (AF164093), c109s (AF164094), Wels/O64 (U95309), Wels/O24 (U95302), JiTr/Opava /12 (KC494908), Moravia/02 (Z49915), Moravia/94 (Z48741), Moravia/86 (Z48573), Moravia/93 (Z48574), Koziky/47 (AJ223600), Koziky/76 (AJ223601), Korneuburg/K11 (U95305), Korneuburg/K26 (U95310), Malacky/370, Malacky/32. Bootstrap values ≥70%, calculated from 1,000 replicates, are shown at the tree branches. Arrow indicates strain isolated in this study. The tree is drawn to scale. The scale bar indicates an evolutionary distance of 0.02 substitutions per position in the sequence.