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. 2014 Mar;133(3):899-901.e3.
doi: 10.1016/j.jaci.2013.09.020. Epub 2013 Nov 5.

Prostaglandin D2 regulates human type 2 innate lymphoid cell chemotaxis

Jinny E Chang  1 , Taylor A Doherty  2 , Rachel Baum  2 , David Broide  3
Affiliations

Prostaglandin D2 regulates human type 2 innate lymphoid cell chemotaxis

Jinny E Chang et al. J Allergy Clin Immunol. 2014 Mar.
No abstract available

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Conflict of interest statement

Disclosure of potential conflict of interest: J. E. Chang claims no relevant conflicts of interest.

Figures

FIG 1
FIG 1
ILC2 FACS: Lymphocytes were identified from whole PBMCs (left panel), and lineage-negative cells gated (middle panel). Lineage-negative lymphocytes were further assessed for the expression of CD127 and CRTH2 (right panel). ILC2 were identified as lineage-negative CD127+ CRTH2+ lymphocytes and represent 12.8% of the cells in this subject (range 1%–20% in all subjects). FACS, Fluorescence-activated cell sorting; FSC, forward scatter; LIN, lineage; SSC, side scatter.
FIG 2
FIG 2
ILC2 chemotaxis to PGD2: PBMCs (Fig 2, A: n = 4 allergic subjects; Fig 2, B: n = 6 nonallergic controls) were incubated in duplicate in the upper chamber of a 5-µm pore-size plate. Varying concentrations of PGD2 were placed in the lower chamber. Each PGD2 concentration (5 nM PGD2, 25 nM PGD2), as well as control 0 nM PGD2, was dissolved in 5% DMSO in PBS. The number of ILC2 in the lower chamber was assessed by using FACS at 90 minutes. DMSO, Dimethyl sulfoxide; FACS, fluorescence-activated cell sorting. *P < .05 and **P < .01.
See this image and copyright information in PMC

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