Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1989 Feb;177(2):261-4.
doi: 10.1007/BF00392814.

Phosphoribosyl pyrophosphate and the measurement on inorganic pyrophosphate in plant tissues

J E Dancer  1 , T Ap Rees
Affiliations

Phosphoribosyl pyrophosphate and the measurement on inorganic pyrophosphate in plant tissues

J E Dancer et al. Planta. 1989 Feb.

Abstract

This work provides further evidence that plants contain appreciable amounts of inorganic pyrophosphate (PPi), and that breakdown of phosphoribosyl pyrophosphate (PPRibP) does not contribute significantly to the PPi detected in plant extracts. Inorganic pyrophosphate in extracts of the roots of Pisum sativum L., clubs of the spadices of Arum maculatum L., and the developing endosperm of Zea mays L. was assayed with pyrophosphate fructose 6-phosphate 1-phosphotransferase (EC 2.7.1.90), and with sulphate adenyltransferase (EC 2.7.7.4). The two different assays gave the same value for PPi content, and for recovery of added PPi. It was shown that PPRibP is converted to PPi during the extraction of PPi. However, the amounts of PPRibP in clubs of A. maculatum and the developing endosperm of Z. mays were negligible in comparison with the contents of PPi.

PubMed Disclaimer

References

    1. J Biol Chem. 1984 Apr 25;259(8):5087-92 - PubMed
    1. Biochim Biophys Acta. 1976 Jun 23;437(1):22-35 - PubMed
    1. Plant Physiol. 1984 Jul;75(3):862-4 - PubMed
    1. Planta. 1984 Sep;162(2):188-91 - PubMed
    1. Plant Physiol. 1985 Sep;79(1):159-64 - PubMed