Bacterial growth on dissolved organic carbon from a blackwater river

Abstract

Different nominal molecular weight (nMW) fractions of DOC from a southeastern blackwater river were concentrated by ultrafiltration and added to sieved river water to assess each fraction's ability to stimulate bacterial growth. Bacterial growth was measured using change in bacterial biomass from direct counts and using(3)H-thymidine incorporated into DNA. Bacterial growth and amount of DOC used was greatest in the low MW enrichment (< 1,000 nMW) and least in the intermediate MW enrichment (1,000-10,000 nMW). The high MW fraction (> 10,000 nMW) supported more growth than did the intermediate MW fraction, apparently because of lower MW compounds complexed with a high MW refractory core. The low MW fraction of DOC from a clearwater mountain stream, a boreal blackwater river, and leachate from water oak and willow leaves also stimulated more bacterial growth than did other fractions. However, the high MW DOC from these other sources was not as biologically available as high MW DOC from a blackwater river. Bacteria converted blackwater river DOC to bacterial biomass with an efficiency of 31%. Bacteria produced at the expense of abundant riverine DOC provide a trophic resource for protozoa and higher levels of the microbial food web of a blackwater river.