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Case Reports
. 2013 Nov-Dec;55(6):429-31.
doi: 10.1590/S0036-46652013000600011.

Usefulness of kDNA PCR in the diagnosis of visceral leishmaniasis reactivation in co-infected patients

Antonio Carlos Nicodemo  1 , Valdir Sabbaga Amato, Felipe Francisco Tuon, Regina Maia de Souza, Thelma Suely Okay, Lúcia Maria Almeida Braz
Affiliations
Case Reports

Usefulness of kDNA PCR in the diagnosis of visceral leishmaniasis reactivation in co-infected patients

Antonio Carlos Nicodemo et al. Rev Inst Med Trop Sao Paulo. 2013 Nov-Dec.

Abstract
in English, Portuguese

It is important to develop new methods for diagnosing relapses in the co-infection of visceral leishmaniasis (VL) and HIV to enable earlier detection using less invasive methods. We report a case of a co-infected patient who had relapses after VL treatment, where the qualitative kDNA PCR showed a good performance. The kDNA PCR seems to be a useful tool for diagnosing VL and may be a good marker for predicting VL relapses after treatment of co-infected patients with clinical symptoms of the disease.

RESUMO: É importante a pesquisa de novos métodos laboratoriais para o diagnóstico de recidivas em casos de co-infecção leishmaniose visceral (LV) e vírus da imunodeficiência humana (HIV), que permitam o diagnóstico precoce das recidivas, utilizando métodos menos invasivos. Descrevemos aqui, o caso de paciente co-infectada que apresentou recidivas após o tratamento da LV e onde a PCR qualitativa demonstrou bom desempenho. A kDNA PCR parece ser ferramenta útil para o diagnóstico de recidivas de LV após o tratamento em pacientes co-infectados com sintomas clínicos da doença.

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Conflict of interest statement

Conflicts of interest statement: The authors have no associations or commercial relationships which might represent conflicts of interest.

Figures

Fig. 1
Fig. 1. Detection of kDNA-120 bp amplification in the sample from bone marrow (BM) and peripheral blood (PB) of the patient with LV.
See this image and copyright information in PMC

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